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KMID : 0525720170220040235
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Journal of Chitin and Chitosan
2017 Volume.22 No. 4 p.235 ~ p.240
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Characterization of Cellulase from Bacillus sp. JB1 from Forest Soil
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Kwon Yoon-Jeong
Choi Ji-In
Kim Sang-Jin
Park Chang-Su
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Abstract
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One cellulolytic microorganism was isolated from forest soil due to its ability of forming active zone on LB-Agar plate containing carboxymethylcellulose (CM-cellulose) with trypan blue. In the analysis of 16S rRNA gene sequence, this strain showed 99% of identity with Bacillus sp.(GeneBank HQ433576). From this result, we named this strain as Bacillus sp. JB1. Bacillus sp. JB1. Optimum pH and temperature of cellulase from Bacillus sp. JB1 was 4.0 and 60°C, respectively. The cellulase was stable from pH 4.0 to 7.0 and up to 60°C for treatment 30 min. In the substrate specificities of cellulase from Bacillus sp. JB1, the cellulase exhibited the highest activity for CM-cellulose among CM-cellulose, Alkali swollen cellulose, a-cellulose, Sigmacell cellulose and Avicel. The cellulase from Bacillus sp. JB1 showed enzyme activity clearly for non-microcrystalline cellulose such as CM-cellulose and Alkali swollen cellulose but did not exhibit for microcrystalline cellulose such as a-cellulose, Sigmacell cellulose and Avicel. In the degradation of 3% CM-cellulose, 5 U/ml of cellulase from Bacillus sp. JBl was determined the most proper enzyme activity.
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KEYWORD
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Forest soil, Bacillus sp., Cellulase, Characterization, Screening
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